Lüchtefeld, Ines

Research Interests:

Forces
in Membrane Biophysics
& Mechanosensitivity

• Combining atomic force microscopy with micropipettes to investigate the forces at lipid membranes during contact and fusion
• Adapting tools to study single-cell mechanosensitivity

Education

• B.Sc. Physics, RWTH Aachen
• M.Sc. Biomedical Engineering, RTWH Aachen

Publications

• external pageLocalized detection of ions and biomolecules with a force-controlled scanning nanopore microscopecall_made
  M. Aramesh, C. Forró, L. Dorwling-Carter, I. Lüchtefeld, T. Schlotter, S.J. Ihle, I. Shorubalko, V. Hosseini, D. Momotenko, T. Zambelli, E. Klotzsch, J. Vörös
  Nature Nanotech, 2019, DOI: 10.1038/s41565-019-0493-z
• external pageDark-Field Microwells toward High-Throughput Direct miRNA Sensing with Gold Nanoparticlescall_made
  S. Hwu, Y. Blickenstorfer, R.F. Tiefenauer, C. Gonnelli, L. Schmidheini, I. Lüchtefeld, B-J. Hoogenberg, A.B. Gisiger, J. Vörös
  ACS Sensors, 2019, doi.org/10.1021/acssensors.9b00946
• external pageForce controlled SU-8 micropipettes fabricated with a sideways processcall_made
  H. Han, V. Martinez, M. Aebersold, I. Lüchtefeld, J. Polesel-Maris, J. Vörös, T. Zambelli
  Journal of Micromechanics and Microengineering 2018 28:095015 DOI: external page10.1088/1361-6439/aac83ccall_made
• Precise localized chemical stimulation using the FluidFM to probe spatio-temporal dynamics of neural networks towards an in vitro stroke model
  G. Thompson-Steckel, M.J. Aebersold, H. Dermutz, I. Lüchtefeld, J.F. Saenz Cogollo, S.-C. Lin, C. Burchert, M. Schneider, H. Han, L. Demkó, T. Zambelli, and J. Vörös
  Conference Proceeding, The 21th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS), 22-26 October 2017, Savannah, USA.
  
• external pageIn Vitro Evaluation of Intra-Aneurysmal, Flow-Diverter-Induced Thrombus Formation: A Feasibility Studycall_made
  K. Gester, I. Lüchtefeld, M. Büsen, S.J. Sonntag, T. Linde, U. Steinseifer, G. Cattaneo
  American Journal of Neuroradiology (2016), DOI: external page10.3174/ajnr.A4555call_made

Oral Presentations

• Investigating the Influence of Membrane Pretension on Single Cell Mechanosensitivity With Force-Controlled Micropipettes
  I. Lüchtefeld, C. Gäbelein, J. Vörös, B. Martinac, T. Zambelli, M. Vassalli
  64th Biophysical Society Annual Meeting, 15-19 February 2020, San Diego, USA, Travel Awardee
• Time- and Force-Controlled Contact between Giant Unilamellar Vesicles and Lipid Bilayers with Simultaneous Fluorescence Intensity Measurement
  I. Lüchtefeld, T. Zambelli, J. Vörös
  EBSA Biophysics Course on Membrane and Lipid-Protein Interactions, 10-15 June 2018, La Grand Motte, France
  
• Manipulation of Single Neurons and Defined Neural Circuits
  I. Lüchtefeld, M.J. Aebersold, V. Martinez, H. Dermutz, L. Demkó, T. Zambelli, J. Vörös
  SSBE Annual Meeting, 30 August 2017, Winterthur, Switzerland
• A Force-Controlled Nanopipette for Single Neuron Manipulation and Adhesion Force Measurement
  I. Lüchtefeld, T. Zambelli, J. Vörös
  Neurofluidics 2017, 23-24 November 2017, Grenoble, France

Poster Presentations

• Force-Controlled Micro-Pipette To Investigate Single-Cell Mechanosensitivity & Membrane Fusion
  I. Lüchtefeld, C. Gäbelein, J. Vörös, T. Zambelli, M. Vassalli
  12th European Biophysics Congress - EBSA, 20-24 July 2019, Madrid, Spain
• Investigation of Forces during Membrane Fusion by using Lipid Vesicles as AFM Probes
  I. Lüchtefeld, T. Zambelli, J. Vörös
  Thematic Meeting of the Biophysical Society on "Quantitative Aspects of Membrane Fusion and Fission", 06-10 May 2019, Padova, Italy
• Investigation of single-cell mechanosensitivity with a force-controlled nano-pipette
  I. Lüchtefeld, C. Gäbelein, B. Martinac, T. Zambelli, M. Vassali
  Nanoengineering for Mechanobiology, 24-27 March 2019, Genova, Italy
• Investigation of Interaction Forces between Lipid Vesicles and Lipid Bilayers with Fluidic Force Spectroscopy
  I. Lüchtefeld, T. Zambelli, J. Vörös
  Biointerfaces International 2018, 14-16 August 2018, Zurich, Switzerland
  
• AFM-based Force Measurements during the Spatiotemporally Controlled Interaction of Lipid Bilayers and Monolayers of Vesicles and Lipid Droplets
  I. Lüchtefeld, T. Weiger, T. Zambelli, J. Vörös
  MaP Graduate Symposium, 26 June 2018, Zürich, Switzerland
  
• Towards the Investigation of Synaptic Vesicle Fusion Mechanisms with Novel Vesicular Force Microscopy
  I. Lüchtefeld, T. Zambelli, J. Vörös
  Biophysical Society 62nd Annual Meeting, 17 - 21 February 2018, San Francisco, USA
• Single Neuron Manipulation with Force-Controlled Nanopipette
  I. Lüchtefeld, M.J. Aebersold, V. Martinez, H. Dermutz, L. Demkó, T. Zambelli, J. Vörös
  FEBS Workshop on Biointerfaces, 2-7 July 2017, Sant Feliu, Spain
• Manipulation of the Neural Cytoskeleton with a Force Controlled Nanopipette
  I. Lüchtefeld, M.J. Aebersold, T. Zambelli, J. Vörös
  NNBE Summer School, 20-26 August 2017, Zermatt, Switzerland
• Investigation of Membrane Vesicle Fusion Mechanisms with Force-Controlled Nanopipette
  I. Lüchtefeld, T. Zambelli, J. Vörös
  NanoBioTech Montreux, 13-15 November, Montreux, Switzerland
• Towards the Investigation of Synaptic Adhesion Forces of Systematically Stimulated Neurons Using Microelectrode Arrays and a Force-Controlled Nanopipette
  Ines Lüchtefeld, Mathias J. Aebersold, Csaba Forro, Greta H. Thompson-Steckel, Serge Weydert, Tomaso Zambelli, Janos Vörös
  Neurofluidics 2017, 23-24 November 2017, Grenoble, France

Supervised Student Projects

• Influence of SNARE Transmembrane Domains on the Forces During Membrane Fusion
  Andreea Mesesan (Internship)
• Immobilization of Giant Lipid Vesicles for AFM-based Biophysical Membrane Measurements
  Nina Mörgeli & Carina Fuss (Group Project)
• New Techniques for Cell Membrane Modification
  Giulia Amirati (Internship)
  
• Investigation of the Forces during the Interaction of Lipid Droplets with Phospholipid Bilayer Membranes using FluidFM
  Theresa Weiger (Bachelor Thesis)